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Purpose:To establishantransplantedEGFPexpressinghepatocellularcarcinomameta-stasismodelinBuffalorats. Materials and Methods:McA-RH7777/EGFPcellsweresubcutaneouslyimplantedintotheBuffaloratsrightthighs.Piecesof tumortissueweretransplantedintotheleftlobesoftheliversin30rats.Thesubsequentgrowth,invasion,metastasisofthe transplantedtumorswereobservedandevaluatedwithwhole-bodyfluorescenceopticalimagingsystem,fluorescentmicroscopy, MRI,CT,DSA.Thetumormarkerswereinvestigatedbyhistologicalanalysis. Results:ThetumorsweredetectedwithMRIinallratsonday7aftertumorimplanted.Themeansurvivaltimewas45.93±5.06 daysinthetumor-bearinggroup.WedemonstratedthatMcA-RH7777/EGFPcellsmaintainedstalbehigh-levelEGFPexpression duringtheirgrowthinvivo.Theinvasionandmetastasisoftumorwerereadilyobservedandaccuratelyevaluatedbyfluorescent microscopy,whole-bodyfluorescenceopticalimagingsystem.Themeantumorvolumeswas12.54±3.89mm3onday7, increasedto201.18±86.39mm3onday14aftertumorimplanted.Thetumordemonstratedwell-demarcatedhypo-intensity signalonT2WIandhyper-intensitysignalonDWI.DSAdemonstratedamasswithnodularorcirculartumorstaining,andwith enlargedortwistedfeedingartery.AFPwaspositive.Pathologically,thetumorswereapproximatelyroundorellipsenodules. Lungmetastasiscanbedetectedinallratsattheendstage.Thisratmodelshavethesimilarimagingandpathological characteristicstothatofhumanHCC. Conclusion:TheEGFPtaggedhepatocellularcarcinomamodelsinBuffaloratsaresuperiorforthedetectionandstudying relevantpatternsofhepatocellularcarcinomainvasionandmetastasisinvivo.Theseratmodelsaresuitableforthestudiesabout imagingdiagnosis,interventionaltherapeutic,tumormetastasisandsoon.