An immuno-rotary biosensor (IRB) was constructed based on FoF1-ATPase with immunological and biochemical techniques to achieve the goal of clenbuterol detection.The main experiments and results are as follows:1.TFIβ subunit from thermophilic bacterium Bacillus PS3 was purified using Ni2+-NTA affinity chromatography and used to immunize BALB/c mice.