Ⅰ型鸭肝炎病毒巢式RT-PCR检测方法的建立及应用

来源 :中英禽病国际研究中心成立典礼暨中英禽病最新研究进展研讨会 | 被引量 : 0次 | 上传用户：sunashelly

【摘要】

：

根据GenBank中Ⅰ型鸭肝炎病毒的基因组序列,应用Oligo6.0软件设计合成了2对特异性引物,建立了Ⅰ型鸭肝炎病毒的巢式RT-PCR检测方法.结果显示,该检测方法具有特异性强、敏感性高、重复性好等优点,可以准确快速检测出极低含量(1pg)的Ⅰ型鸭肝炎病毒.本研究解决了该病在组织病料中检出困难的难题,为工型鸭病毒性肝炎的快速诊断、净化及深入研究奠定了基础.

【作者】

：

李娇王金良祖立闯赵金花沈志强

【机构】

：

山东绿都生物科技有限公司,山东滨州 256600 山东绿都生物科技有限公司,山东滨州 256600

【出处】

：

中英禽病国际研究中心成立典礼暨中英禽病最新研究进展研讨会

【发表日期】

：

2015年6期

【关键词】

：

Ⅰ型鸭肝炎病毒巢式反转录聚合酶链反应基因组序列

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Ⅰ型鸭肝炎病毒巢式RT-PCR检测方法的建立及应用

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