The expression and purification of the recombination protein TAT-msvT34A consisting of mouse survivinT34A mutant fused with TAT cell-penetrating peptide and its transduction efficiency in several tumor and non-tumor cell lines were investigated.In this study, the prokaryotic expression vector pTAT-msvT34A was transformed into expression strain of E coli BL21-DE3 and subsequently the transformed bacteria were induced by IPTG for expression of the recombinant protein.