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γ-aminobutyric acids (GABA) function as principal inhibitory neurotransmitters and play an important role in auditory pathway.GABAB receptor (GABABR) is associated with intracellular signal transduction pathways including protein kinase C (PKC).The present study was designed to examine the distribution and colocalization of glutamic acid decarboxylase 67 (GAD67, the chemical marker for the GABA-containing neuron),GABABR and PKC subunit (PKCγ) in the neurons of the cochlear nucleus complex (CNC)by employing transgenic GAD67-green fluorescent protein (GFP) knock-in mice and time-mating C57BL/6J inbred mice.immunofluorescent histochemistry combined with electron microscopy analysis indicated that about 59.49% of GFP-labeled GABAergic neurons colocalized with GABABR and P PKCγ.in the superficial layer of the dorsal division of the CNC (DCN) and also received afferent projection fibers from other GABAergic neurons.Our results provide morphological evidence that GABABR and PKCy are colocalized in GABAergic neurons in the distinct region of DCN, indicating that local inhibitory effects mediated by GABA probably act on PKCy signal transduction through GABABR in the modulation of auditory information transmission.