The half-smooth tongue sole (Cynoglossus semilaevis, hereafter, tongue sole) is a marine flatfish with great commercial importance for fisheries and aquaculture in China.It has also been a promising model for the study of sex determination mechanisms in fish.Here, we report the construction of a genetic linkage map for the tongue sole, based on 137 markers including 103 AFLP markers, 33 microsatellite markers, and one female-specific DNA marker.Twenty-six linkage groups (LGs) were found.The total map length was 934.6 cM (Kosambi), with an average spacing of 8.4 cM, covering 64.4% of the estimated genome size.Furthermore, a female-specific SCAR marker, CseF-382, was mapped on LG5.This study represents the first genetic linkage map in the tongue sole.This map has great potential in the identification of quantitative traits loci and sex-related genes and marker-assisted selection in the tongue sole.Meanwhile, the new set of polymorphic microsatellite markers developed in this study is not only useful for genetic mapping but also of critical importance for studies on genetic diversity and broodstock management in tongue sole.