母源BCAS2在小鼠早期胚胎发育中的功能

来源 :2012全国发育生物学大会 | 被引量 : 0次 | 上传用户：wpsl5168

【摘要】

：

【作者】

：

徐倩华王凤超张小欣高绍荣李磊

【机构】

：

北京中国科学院动物研究所,计划生育生殖生物学国家重点实验室,100101

【出处】

：

2012全国发育生物学大会

【发表日期】

：

2012年8期

下载到本地 , 更方便阅读

下载此文赞助VIP

声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架

论文部分内容阅读

　　合子基因组激活之前,动物早期胚胎的RNA转录活性很低,RNA的代谢主要表现为母源RNA的降解,功能RNA的剪切活性很低,随合子基因组激活,RNA的剪切活性明显增强.通过RNA剪切体加工前体RNA是所有真核生物的重要基因表达调控方式之一,前体RNA在哺乳动物早期胚胎细胞中加工的分子机制目前仍然不清楚.BCAS2 (Breast cancer amplified sequence 2)最初在研究RNA剪切体组分时被发现,此后在人类乳腺癌细胞系中克隆出来.BCAS2与CDC 5L、Prp19、PLRG1等其他几种RNA剪切因子形成CDC 5L(Cell Division Cycle 5-like)/Prp19 (Pre-mRNA-processing factor 19)蛋白复合体的核心组分,研究发现该蛋白复合体广泛存在于很多细胞系中,通过调节RNA剪切体和DNA损伤修复等多种信号通路行驶重要功能.CDC 5L /Prp19复合体中组分PRP19和PLRG1的基因敲除导致小鼠胚胎致死提示该蛋白复合体及复合体中其他组分在小鼠早期胚胎发育过程中具有重要功能.结论：本研究利用条件性基因敲除小鼠技术证明母源性BCAS2在哺乳动物早期胚胎发育过程中通过调控CDC 5L/Prp19复合体其他组分的稳定及DNA损伤修复路径行驶重要功能.

其他文献

Transgenic expression of recombinant hunman aFGF from Camelina sativa seeds

会议

Foxc1a is essential for zebrafish myogenesis through controlling fgf8 expression

Foxc1a, a fork head transcription factor, has been shown to be required for vertebrate somitogenesis.However, little is known about the molecular mechanism offoxc1a in myogenesis.Knocking down foxc1a,

会议

Understanding the mechanisms of Cytoplasmic incompatibility induced by Wolbachia in Drosophila melan

Wolbachia are obligate endosymbiotic bacteria that infect numerous species of arthropods and nematodes.Wolbachia can induce several reproductive phenotypes in their insect hosts, such as Cytoplasmic i

会议

Roof plate-specific spondin 3 antagonizes Wnt/β-catenin signaling pathway in zebrafish embryos

The Roof plate-specific spondin 3 (RSPO3) plays crucial roles during embryogenesis by promoting Wnt/β-catenin signaling pathway in amphibian and mammals.However, the role of Rspo3 in other vertebrate

会议

Stat3 signaling target Efemp2 controls the assembly of Fibronectin and Laminin for gastrula anterior

Fibronectin and Laminin are the principal components of extracellular matrix, and the assembly into fibrils to form basement membrane is required for cell migration.In embryos, the assembly of Fibrone

会议

Kctd10 regulates heart morphogenesis by repressing the trans-activity of Tbx5

T-box (Tbx) transcription factors play essential roles in cardiac development in vertebrate.In particular, Tbx5 is the key regulator for the formation of cardiac chambers.Tbx5 is a dosage-dependent tr

会议

Klf4 is required for germ layer differentiation and body axis patterning during Xenopus embryogenesi

Klf4 is a transcription factor in the family of Kruppel-like factors and plays important roles in stem cell biology, however, its function during embryogenesis is unknown.Here, we report the character

会议

Early Embryonic Lethality of Mice Lacking BCAS2

BCAS2 gene is initially identified as an up-regulated gene in MCF-7 and BT-20 breast cancer cell and one of the core component in CDC5L/PRP19 complex, which are an evolutionarily conserved splicing co

会议

Diversification of the expression patterns and functions of the Frizzled gene family during chordate

The Wnt signaling pathway has been reported to play essential roles in embryonic patterning among all the examined metazoans.Recent studies on Wnt genes in the basal chordate amphioxus embryogenesis h

会议

SCMC regulates the actin cytoskeleton dynamics that play important role on the function of spindle d

Although maternal effect genes play critical roles in early embryogenesis, their molecular functions in mammals remain largely unknown.Using mouse genetics and biochemistry, we have identified a mamma

会议

母源BCAS2在小鼠早期胚胎发育中的功能

与本文相关的学术论文