Complete sequence and gene organization of the mitochondrial genome of Bean goose (Anser fabalis sib

来源 :第八届全国野生动物生态与资源保护学术研讨会 | 被引量 : 0次 | 上传用户:kathrynde
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  In the present study,we determined the complete mitochondrial genome of the Bean goose using overlapping polymerase chain reaction (PCR) amplification (GenBank accession no.HQ890328).The genome was 16 688 bp in length,containing 13 protein-coding genes (ATP6,ATP8,COI-Ⅲ,ND1-6,ND4L,Cyt b),2 ribosomal RNAs (12S rRNA and 16S rRNA),22 transfer RNAs and a putative control region (D-loop).The Bean goose mtDNA genes were in the same order and orientation similar to other Anseriformes species.All protein-coding genes started with an ATG codon except the Cyt b,ND5,COI and CoII,TAA was the most frequent stop codon.All tRNA genes can be folded into canonical cloverleaf secondary structure except for tRNASer(AGY) and tRNALeu(CUN),which lose "DHU" arm.There was only one control region in Bean goose mtDNA,some conserved sequence boxes such as F,E,D,C were identified in control region of the control region.Based on the concatenated nucleotide sequences of mtDNA genes (Cyt b and ND2),we reconstructed phylogenetic trees and discussed the phylogenetic relationships among 36 Anseriformes species.The results show that the Mergini is a non-monophyletic group,our results also clearly suggests the Somateria forms to be a monophyletic in its own tribe Somateirini.Our molecular results suggest Dendrocygna (Dendrocygna javanica) and Anseranatidae (Anseranas semipalmata) are not belong to Anserinae,but represent to be an independent subfamily which placed inside the family Anatidae.
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