Capillary monolithic columns have been widely applied in capillary liquid chromatography since the easy preparations,unique properties and high performances,which have been suggested as the great alternatives to the particulatepacked and open-tubular capillary columns for separation sciences.Based on chemistry nature of monomers applied,the monolithic columns can be classified into the organic polymer-based and the inorganic silica-based monolithic columns.In recent years,we have developed the “one-pot” strategy to prepare the organic-silica hybrid monolithic columns,by integrating the organic functional moieties into the inorganic silica matrices.With this strategy,a variety of organic-silica hybrid monolithic capillary columns including hydrophobic and hydrophilic have been thus prepared afterwards.Herein,we are offering a new method to prepare the organicsilica hybrid monolithic column for capillary liquid chromatography with high column efficiency,by using the silica nanoparticles as the seeds and/or building blocks to tune the sol-gel growth of the organic-silica hybrid monolithic columns.