Two hundred and forty-nine soil samples were collected from 16 provinces in Thailand on October 2008 to May 2009.Alcohol and heat treatment methods and Gochenaurs glucose ammonium nitrate agar were employed.Identification of fungal isolates was based on morphological characteristics, as colony growth pattern, color and texture on standard media.Microscopic characteristics comprising Aspergillus anamorphic state, size, shape and ornamentation of ascospores were examined under light,stereo and scanning electron microscopes.