Damage to the central nervous system (CNS) results in a glial reaction,leading eventually to the formation of a glial scar.Reactive astrocytes are the major components of glia scar.Oligodendrocyte precursor cells,briefly OPCs,are the fifth element in the CNS,in addition to neurons,astrocytes,oligodendrocytes and microglia.However,there has not been much research on the reaction of OPCs in CNS injury.Researches indicated that many experimental brain injury can arouse the proliferation of NSC in distant SVZ and SGZ,and these cells have the ability to differentiate into astrocyes as well as neurons.To investigate the cell proliferating effects after central nervous system injury,we use unilateral eye ball enucleation of adlut rats as the CNS injury model.And then detect the changes of GFAP,NG2 IR cells in contralateral superior colliculus at different time points.In addition we use BrdU labelling method to detect the proliferation of BrdU IR ceils in the injury place,dentate gyrus and SVZ.Combined with BrdU/GFAP,BrdU/DCX immunofluorescence double labelling,we further investigate the differentiation of the proliferating cells.