【摘 要】
:
Objective: To establish the immortalized oral mucosal epithelial (OME) cell line by transfection with human telomerase reverse transcriptase (hTERT).Methods: Oral mucosal epithelial cells were infecte
【机 构】
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Department of Oral and Maxillofacial Surgery,Nanfang Hospital
【出 处】
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11th Asian Congress on Oeal and Maxillofacial Surgery(第十一届亚洲
论文部分内容阅读
Objective: To establish the immortalized oral mucosal epithelial (OME) cell line by transfection with human telomerase reverse transcriptase (hTERT).Methods: Oral mucosal epithelial cells were infected with retrovial vector encoding hTERT.Infected cells were selected with G418 and checked by immunocytochemistry (ICC), in vitro proliferation, the ability of tumorigenesis, RT-PCR and Western blot detect the expression ofhTERT.
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