Zebrafish as a vertebrate animal model is increasingly used for in vivo drug discovery and for assessing compound toxicity and safety, for its several compelling experimental advantages including transparency, high throughput,short test period, low cost, small amount of compound required, easy manipulation and so on.Traditional Chinese medicine usually are complex mixtures comprising of multicomponent and have multi-targets, the traditional in vitro and in vivo drug research approaches are not very suitable for TCM.