目的构建22型人类血小板抗原(HPA)基因分型参考品(质控DNA),并对其进行测序鉴定。方法提取人血液基因组DNA,以其为模板,通过特异性引物扩增出各HPA(1a—16a)基因片段,胶回收后克隆至pUCm-T载体,随后转化E.coli DH5α,提取质粒酶切鉴定并测序;再以构建成功的HPA-1a—16a质粒为模 Objective To construct a genotyping reference (control DNA) of type 22 human platelet antigen (HPA) and identify it by sequencing. Methods Genomic DNA of human blood was extracted and used as a template to amplify the HPA (1a-16a) gene fragments by specific primers. The recovered fragments were cloned into pUCm-T vector and subsequently transformed into E. coli DH5α. Identified and sequenced; and then constructed successfully HPA-1a-16a plasmid as a model