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  5. sRNA341与kitH相互作用调节变异链球菌耐酸特性的研究

sRNA341与kitH相互作用调节变异链球菌耐酸特性的研究

来源 :中华口腔医学会第十一次全国牙体牙髓病学学术大会 | 被引量 : 0次 | 上传用户：dylwq
【摘 要】
：
目的：探究变异链球菌中small RNA 与其靶基因的相互作用关系,及其对变异链球菌各种表型(生存曲线、耐酸、生物膜)的影响.方法：① RNA 深度测序变异链球菌UA159 菌株,筛选出30 个候选sRNA,使用targetRNA2 软件预测各自靶基因；②Northern blotting 验证sRNA341 存在；③采用IFDC2 基因无痕敲除方法、以穿梭质粒pDL278 为基因载体,构建靶基因
【作 者】
：
罗玉 郭强 彭显 徐欣 周学东 
【机 构】
：
口腔疾病研究国家重点实验室,国家口腔疾病临床医学研究中心,四川大学华西口腔医院牙体牙髓病科,成都,610041
【出 处】
：
中华口腔医学会第十一次全国牙体牙髓病学学术大会
【发表日期】
：
2018年11期
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　　目的：探究变异链球菌中small RNA 与其靶基因的相互作用关系,及其对变异链球菌各种表型(生存曲线、耐酸、生物膜)的影响.方法：① RNA 深度测序变异链球菌UA159 菌株,筛选出30 个候选sRNA,使用targetRNA2 软件预测各自靶基因；②Northern blotting 验证sRNA341 存在；③采用IFDC2 基因无痕敲除方法、以穿梭质粒pDL278 为基因载体,构建靶基因kitH 突变株S.mutans ΔKitH、回复株ΔKitH/pDL278-KitH、sRNA341 过表达株S.mutans pDL278-RNA341；④qPCR 验证S.mutans pDL278-RNA341 中kitH 基因表达；⑤采用生长曲线、结晶紫染色、耐酸实验等检测野生株与各突变株的表型变化.⑥qPCR 验证野生株在低pH 环境刺激下,sRNA341 及kitH 转录水平上的相对表达变化.
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