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目的探讨止消通脉宁对系膜细胞（ＭＣ）增殖的影响。方法采用血清药理方法，提取动物的含药血清作用于体外高糖培养的人系膜细胞（ＨＭＣ）体系，观察了具有益气养阴、化瘀散结作用的止消通脉宁对ＨＭＣ增殖的影响。结果止消通脉宁具有抑制高糖培养的ＨＭＣ增殖作用，该抑制作用具有一定的量效关系；治法拆方研究表明，益气养阴组分和化瘀散结组分均具有抑制高糖培养的ＨＭＣ增殖作用，且呈一定的量效关系，二者合用有一定协同作用。结论ＭＣ是止消通脉宁发挥治疗作用的重要靶细胞，抑制ＭＣ增殖可能是止消通脉宁防治糖尿病肾病（ＤＮ）的重要机理之一。
Objective To investigate the effect of Zhixiao Tongmaining on the proliferation of mesangial cells (MC). METHODS Serum pharmacological methods were used to extract the animal serum containing methotrexate in human mesangial cells (HMC) cultured in high glucose in vitro. The effects of Zhixiao Tongmaining on the proliferation of HMC with the effect of supplementing Qi, nourishing yin, and removing stasis were observed. Impact. Results Zhixiao Tongmaining had the effect of inhibiting the proliferation of HMC cultured in high-glucose culture. The inhibitory effect had a certain dose-effect relationship. The study of dismantling methods showed that the components of Yiqi Yangyin and Huayu Sanjie had high inhibition. The proliferation of HMC in the culture of sugar was in a certain dose-effect relationship, and there was a certain synergistic effect in the combination of the two. Conclusion MC is an important target cell for the therapeutic effect of Zhi Xiao Tong Mai Ning. Inhibiting MC proliferation may be one of the important mechanisms of Zhi Xiao Tong Mai Ning in the prevention and treatment of diabetic nephropathy (DN).