论文部分内容阅读
In this study, we demonstrated selenium (Se) accumulation in Bifidobacterium longum strain (B.longum) and evaluated the effect of Se-enriched B.longum (Se-B.longum) on tumor growth and immune function in tumor-bearing mice.Analysis using high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) revealed that more than 99% of Se in Se-B.longum was organic Se the main component of which was selenomethionine (SeMet).In the in vivo experiments, tumor-bearing mice (n=8) were orally administrated with different doses of Se-B.longum alone or combined with cyclophosphamide (CTX).The results showed that the middle and high dose of Se-B.longum significantly inhibited tumor growth.When Se-B.longum and CTX were combined, the antitumor effect was significantly enhanced and the survival time of tumor-bearing mice (n=12) was prolonged.Furthermore, compared with CTX alone, the combination of Se-B.longum and CTX stimulated the activity of natural killer (NK) cells and T lymphocytes, increased the levels of intedeukin-2 (IL-2) and tumor necrosis factor-α (TNF-α), and the leukocyte count of H22 tumor-bearing mice (n=12).