【摘 要】
：
本文以猕猴桃病毒病为研究对象，对侵染猕猴桃的病毒种类进行了鉴定，已发现的病毒有猕猴桃病毒A(Actinidia virus A，AcVA)、猕猴桃病毒B(Actinidia virus A，AcVB)和苹果茎沟病毒(apple stem grooving virus,ASGV)。同时采用siRNA测序技术鉴定出一种侵染猕猴桃的欧洲山棒环斑病毒属(Emaravirus)新病毒，根据拼接序列设计引物扩
【机 构】
：
华中农业大学植物科学技术学院,武汉430070
【出 处】
：
中国植物病理学会2014年学术年会
论文部分内容阅读
本文以猕猴桃病毒病为研究对象，对侵染猕猴桃的病毒种类进行了鉴定，已发现的病毒有猕猴桃病毒A(Actinidia virus A，AcVA)、猕猴桃病毒B(Actinidia virus A，AcVB)和苹果茎沟病毒(apple stem grooving virus,ASGV)。同时采用siRNA测序技术鉴定出一种侵染猕猴桃的欧洲山棒环斑病毒属(Emaravirus)新病毒，根据拼接序列设计引物扩增获得其全基因组序列，其基因组由4条负义单链RNA组成，各RNA分别含1个开放式阅读框，其中RNA1大小为7061个核苷酸(nt)，推测编码2303个氨基酸的复制相关蛋白；RNA2由2267个核苷酸组成，编码含653个氨基酸的糖蛋白；RNA3为1678个核苷酸，编码310个氨基酸的核衣壳蛋白；RNA4大小为1664个核苷酸，编码379个氨基酸的功能未知蛋白。该病毒的RNA1～4与redbud yellow ringspot virus(RYRV)相应RNA相似性最高，分别为57.5％(RNA3)—65.7%(RNAl)，所推测的编码蛋白相似性分别为65.1%、48.3%、55.7%和58.9%；且在各RNA的3’和5’端均具有由15个nt和17个nt组成的高度保守的反向重复序列。根据其序列特点，初步确定该病毒为Emaravirus属的一个新的病毒种。
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