The first cooperated effort of the Chinese Human Chromosome Proteome Consortium (CCPC) last year aimed to produce an extreme huge dataset,and got about 59.8 ％coverage against all the protein-coding genes.In order to explore the proteome issues in a detailed way,more elaborate experiments were designed which performed a deep profiling for transcriptome,translatome and proteome with the same batch of cultured hepatoma cells (Hep3B,MHCC97H and HCCLM3).