【摘 要】
：
Glycosylation is one of the most common post-translational modification of proteins, and plays a major role in a variety of biological activities, e.g.receptor-ligand interaction, signal transduction
【机 构】
：
Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R.& A.Center
【出 处】
：
第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
论文部分内容阅读
Glycosylation is one of the most common post-translational modification of proteins, and plays a major role in a variety of biological activities, e.g.receptor-ligand interaction, signal transduction and immune response [1, 2].Global profiling of glycosylation sites and structural determination of glycans are important prerequisite for advanced investigation of the biological functions of glycoproteins.However, the analysis of glycopeptides directly from proteolytic digests is as well frequently obstacle to the low ionization efficiency of glycopeptides and low glycopeptide signal among the large excess nonglycosylated peptides [3].Therefore, exploring a selective glycopeptide enriching method followed by MS techniques, becomes more imperative.
其他文献
Bisphenol A (BPA) among the groups of endocrine disrupting chemicals (EDCs) has aroused growing concerns because of their carcinogenicity and ubiquitousness.Herein, dispersive liquid-liquid microextra
We focus a monolithic silica as a new sorbent for sample extraction of drugs in biological materials.The monolithic silica disk was prepared and mounted into spin column.In spin column extraction, the
Leonurine, one of the major active ingredients from Herba leonuri, has been considered as a good drug candidate.However, it is difficult to isolate and purify leonurine from natural resources due to t
Polycyclic aromatic hydrocarbons (PAHs) as a class of important persistent organic pollutants (POPs) have been increasingly investigated.Herein, a novel method of molecularly imprinted solid-phase ext
Ultrasound-assisted emulsification microextraction was applied for the extraction and preconcentration of the sulfonylurea herbicides from orange juice sample prior to their determination by HPLC with
In recent research of proteome, isolation and detection of biomarkers from complex biological samples, such as serum, urea, cerebrospinal fluid and so on, are of great significance.But, the fact is th
A partition coefficient is a parameter reflecting hydrophobicity of chemical regents and is commonly assessed as the characteristic factor which affects the bio-availability of the chemical compound i
Solid-phase extraction (SPE) has been widely used to purify and enrich analytes of interest from complex samples.Among various sorbents, the mixed-mode sorbent seems to be more promising than others s