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Protein derived from sheep-bone was hydrolyzed by papain.Response surface methodology was used to optimize the hydrolysis conditions,like temperature,substract concentrate,enzyme to substract and hydrolysis time.The results showed the optimum conditions were pH7.0,temperat-ure 64.05℃,enzyme to substrate 1576U/g,hydrolysis time 7.22h,substrate concentration 0.27 tkg/L.