论文部分内容阅读
Objectives To study the expressions of endothelin-1 at early stages of atrial fibrillation in a rapid paced primary cultured atrial myocyte model.Methods Primary rat atrial myocytes were cultured and a rapid paced cell model was established.The polymerase chain reaction (RT-PCR) and Enzyme-Linked Immunosorbent Assay (ELISA) were applied to detect the messenger ribonucleic acid (mRNA) expression and secretion of endothelin-1.