EXPRESSIONS OF ENDOTHELIN-1 IN PRIMARY CULTURED ATRIAL MYOCYTES AFTER RAPID ELECTRICAL-FIELD STIMULA

来源 :第二十四届长城国际心脏病学会议、亚太心脏大会暨国际心血管病预防与康复会议(The 24th Great Wall Int | 被引量 : 0次 | 上传用户:ck101newguy
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Objectives To study the expressions of endothelin-1 at early stages of atrial fibrillation in a rapid paced primary cultured atrial myocyte model.Methods Primary rat atrial myocytes were cultured and a rapid paced cell model was established.The polymerase chain reaction (RT-PCR) and Enzyme-Linked Immunosorbent Assay (ELISA) were applied to detect the messenger ribonucleic acid (mRNA) expression and secretion of endothelin-1.
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