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We have been investigating a new concept of chromatography, a temperature-responsive chromatography, using a temperature-responsive poly (N-isopropyl acrylamide) (PNIPAAm) modified surface for the packing materials of chromatography.PNIPAAm-grafted surfaces showed temperature-responsive hydrophilic-hydrophobic surface-property alterations.Using these features, PNIPAAm and related temperature-responsive polymers were used to generate temperature-sensitive stationary phases for chromatographic systems.We modified several different functional polymers to silica beads, including temperature and pH-responsive polymers.Temperature-dependent chromatographic interactions with soluble analytes were modulated by changing the grafted PNIPAAm chain property with a constant aqueous media as the mobile phase.The applications of thermally responsive columns have been demonstrated for separations in the HPLC mode.Temperature-responsive chromatography is performed with an aqueous mobile phase without using an organic solvent, such as methanol or acetonitrile.For example, we achieved temperature-dependent separations of steroids and amino acids using only water as a mobile phase.