【摘 要】
：
Swine mycoplasmal pneumonia (SMP), caused by Mycoplasma hyopneumoniae (Mhp), is one of the most important respiratory diseases in swine breeding.The objecti
【机 构】
：
ChinaInstituteofVeterinarydrugControl,KeylaboratoryofVeterinaryBiologicsDevelopmentandEvaluationofMO
【出 处】
：
The 6th Meeting of the Asian Organization for Mycoplasmology
论文部分内容阅读
Swine mycoplasmal pneumonia (SMP), caused by Mycoplasma hyopneumoniae (Mhp), is one of the most important respiratory diseases in swine breeding.The objective of this study was to develop a recombinant subunit vaccine rLTBR1 containing R1 region of p97 adhesin of M.hyopneumoniae (Mhp) and B subunit of the heat-labile enterotoxin of Escherichia coli (LTB).The result indicated that rLTBR1 revealed GM1 ganglioside-binding capacity.The immunogenicity of rLTBR1 was detected through BALB/ c mice.The results suggested that inoculated rLTBR1 through the intranasal (IN) or intrasubcutaneous (IS) route both produced specified anti-Mhp systemetic and mucosal antibody (sIgA).The group inoculated R1 by IS route also produced specified anti-Mhp systemetic antibody, but through the IN route didnt induce specified and mucosal antibody.Group administrated rLTBR1 through the IN route also induced IFN-γ secretion by lymphocytes.Howerver, the other groups didnt induced IFN-γ compared to the negative group.In conclusion, LTB can enhance the mucosal and cellular immunity level through the IN route.Certainly, the immune responses of mice should not be extrapolated, so the potential of the rLTBR1 for the control of SMP requires further studies in pigs.This study suggested that rLTBR1 vaccine may establish a new strategy for preventing infection from M.hyopneumoniae.
其他文献
我国尚无平滑肌肉瘤接受曲贝替定治疗的报道.1例54岁女性患左大腿复发性平滑肌肉瘤伴肺内单发转移,左大腿局部病变及肺内病灶经4次MAID方案化疗后仍进展,患者随后接受了4
Hydroxysafflor yellow A (HSYA), the main active pharmaceutical ingredient of the safflower plant (Carthamus tinctorius L.), is a hydrophilic drug with low o
脂蛋白作为一种内源性纳米颗粒，具有良好的生物相容性、可彻底生物降解性、无免疫原性，不被体内网状内皮系统识别而快速清除，是一种极具前景的靶向药物载体。近年来基于脂蛋
Vasodegenerative disease, like other so-called diseases of aging, begins decades before it becomes clinically apparent.The main underlying pathologies are a
Reduced levels of neurogenesis and microvasculature, and chronic lowlevel inflammation in the form of astrocyte hypertrophy and activated microglia are amon
The proposal is aimed to develop the ELISA diagnostic reagents for large-scale screening of mycoplasma mastitis in bovine herds.Six clones, IC6, IE4, IB8, I
Spiroplasmas is an evolutionarily interesting group of bacteria belonging to the class Mollicutes.They have small size and helical structure, lack of a cell
Mycoplasma hyopneumoniae is causative agent of swine mycoplasmal pneumonia worldwide.Most of M.hyopneumoniae vaccine components are inactivated bacterin.Dev
The importance of information theory methods for aging research has been recognized for over half a century (Yockey et al.1958).The information theoretical
There are two Ureaplasma species (14 serovars), U.parvum and U.urealyticum, found in humans as common colonizers of adult urogenital tracts.However, they are al