【摘 要】
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Objective: To investigate whether endoplasmic reticulum (ER) stress participates in the induction of apoptosis in HepG2 cells exposed to high glucose and the me
【机 构】
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LaboratoryofMolecularBiology,AnhuiMedicalUniversity
【出 处】
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2014年第十二届全国脂质与脂蛋白学术会议
论文部分内容阅读
Objective: To investigate whether endoplasmic reticulum (ER) stress participates in the induction of apoptosis in HepG2 cells exposed to high glucose and the mechanisms underlying the progression of this apoptosis.Methods: A series of experiments were performed following HepG2 cells treated with different concentrations of glucose for 48h.The apoptosis was detected with Hoechst staining and flow cytometry.Caspase-3 activity was performed by measuring the pNA (p-nitroaniline) to indirectly reveal the catalytic activity of caspase-3.The expression levels of apoptosis-, ER stress-associated proteins and MAPKs under the condition of high glucose were analyzed by western blot.To further characterize the molecular mechanisms, the effects of antioxidant alpha-lipoic acid (ALA) and specific inhibitors for JNK and p38 (SP600125 and SB203580, respectively) were examined by hoechst staining, immunofiuorescence and western blot.
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