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The effects of myricetin on 6-hydroxydopamine induced neurodegeneration in the substantia nigra-striatum system were investigated.By high-performance liquid chromatography electrochemical detection,we showed that the dopamine content in the striatum decreased after 6-hydroxydopamine treatment,which could be restored by myricetin.The immunohistochemistry and semi-quantitative RT-PCR studies showed that myricetin could prevent the 6-hydroxydopamine-induced decrease of tyrosine hydroxylase positive neurons and the tyrosine hydroxylase mRNA expression in the substantia nigra.Perls iron staining study further demonstrated that myricetin prevented the 6-hydroxydopamine-induced increase of iron-staining cells in the substantia nigra.These results suggested that the protective effects of myricetin on the toxicity of 6-hydroxydopamine could be attributed to the myricetin-suppressed iron toxicity.