【摘 要】
：
为了建立测定环黄芪醇大鼠血药浓度的HPLC-MS方法并研究其在大鼠体内的药代动力学.采用蛋白沉淀法制备大鼠血浆样品；色谱和质谱条件：Agilent TC-C18(4.6×150mm,5μm)色谱
【机 构】
：
北京中医药大学中药学院,北京100102北京美迪克斯生物技术有限公司北京100012哈尔滨商业大学生命科学与环境研究中心,黑龙江哈尔滨150028
【出 处】
：
第八届全国微全分析系统学术会议、第三届全国微纳尺度生物分离分析学术会议暨第五届国际微化学与微系统学术会议
论文部分内容阅读
为了建立测定环黄芪醇大鼠血药浓度的HPLC-MS方法并研究其在大鼠体内的药代动力学.采用蛋白沉淀法制备大鼠血浆样品；色谱和质谱条件：Agilent TC-C18(4.6×150mm,5μm)色谱柱,流动相为乙腈-0.1％甲酸(65：35),吴茱萸内酯作内标,在三重四极杆串联质谱仪上,采用电喷雾离子化(ESI),正离子方式SIM 模式进行监测；大鼠分别单次灌胃环黄芪醇混悬液10,20和40mg·kg-1三个剂量组后,按时间点取血,HPLC-MS 法测定其血药浓度.实验数据用WinNonlin4.1软件采用非房室模型计算主要药动学参数.结果：环黄芪醇在25.5～1632ng·mL-1内线性关系良好(r=0.9989),定量限为25.5 ng·mL-1,准确度(RE)在±1％内,日内、日间精密度(RSD)均小于6％.三个剂量组主要药代动力学参数：Tmax 分别为0.11+0.09,0.15+0.09,0.10+0.04h,Cmax分别为162.95+50.40,264.52+151.27,552.30+69.88 ng·mL-1,AUC0～t 分别为853.08+243.48,1679.26+1065.99,3524.07+771.20hr·ng·mL-1.环黄芪醇三个给药剂量组的达峰时间基本不变,Cmax和AUC0～t的增加呈剂量依赖特征.本方法灵敏度高、专属性强,适用于环黄芪醇的药代动力学研究.环黄芪醇的药代动力学数据可为临床研究提供依据.
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