【摘 要】
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The expression and purification of the recombination protein TAT-msvT34A consisting of mouse survivinT34A mutant fused with TAT cell-penetrating peptide and
【机 构】
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School of Medical Lab Science, Wenzhou Medical College, China
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The expression and purification of the recombination protein TAT-msvT34A consisting of mouse survivinT34A mutant fused with TAT cell-penetrating peptide and its transduction efficiency in several tumor and non-tumor cell lines were investigated.In this study, the prokaryotic expression vector pTAT-msvT34A was transformed into expression strain of E coli BL21-DE3 and subsequently the transformed bacteria were induced by IPTG for expression of the recombinant protein.
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