【摘 要】
：
Stretch-activated chloride currents (IC1,sA) have been considered to be a component of volume-activated chloride currents (ICl.vol) for some time.This is due to a similarity in biophysical and pharmac
【机 构】
：
Guangdong Key,Laboratory for Bioactive Drugs Research and Department of Biology,Guangdong Pharmaceut
【出 处】
：
The 3rd Ion Channel Conference: Ion channels-Structure, Func
论文部分内容阅读
Stretch-activated chloride currents (IC1,sA) have been considered to be a component of volume-activated chloride currents (ICl.vol) for some time.This is due to a similarity in biophysical and pharmacological properties that involve a membrane curvature-induced mechanism and rearrangement of the cytoskeleton induced by cell swelling or membrane stretch.In the present study, we demonstrated that current density, along with the time taken from the activation of currents to the peak, were significantly different between the two currents, in highly metastatic human hepatocellular carcinoma cells.In addition, the activation of ICl,vol or ICl,SA, induced maximally by hypotonic solutions or membrane stretch, respectively, did not affect the following activation of the other one.Moreover, neither inhibition of IC1,vol by sh-ClC-3 transfection, nor functional blocking of ICl,vol by intracellular dialysis of anti-ClC-3 antibody had an effect on the activation and properties of ICl,SA.Collectively, our results suggest that ICl,SA is different from ICl,vol in activation mechanism and/or in molecular entity responsible for formation of the currents.ClC-3 is involved in the activation of ICl,vol, but not of ICl,SA.
其他文献
Interstitial Cells of Cajal (ICCs) were considered as the pacemaker cells in gastrointestinal(GI) tract.We isolated ICCs from the small intestine of Balb/C mouse and measured the membrane potential an
Vascular endothelial cells respond to blood flow-induced shear stress by generating vaso-relaxing factors such as NO, which prevents abnormal vaso-constrictions and maintains vessels in a relatively d
Objective: To evaluate changes of expression in spinal dorsal horn of rat chronic post-thoracotomy pain (CPTP) model.Methods: 20 adult male Sprague-Dawley rats, weighing 250 ~350g, were randomly divid
Store-operated calcium entry (SOCE) is mediated through the specific plasma membrane channels in response to the depletion of Ca2+ from intracellular Ca2+ stores.TRPC1, STIM1 and Orail may involve in
TRPV4 is a Ca2+-permeable channel and is sensitive to osmotic and mechanical stimuli as well as thermal stimuli.TRPV4 protein expresses widely in various kinds of tissues.In the cardiac myocytes, TRPV
Transient receptor potential melastatin 2 (TRPM2) channel fulfills an important role in oxidative stress signaling in immune and other cells, to which local extracellular acidosis is known to occur un
TRPV4 is a Ca2+-permeable channel and is sensitive to osmotic and mechanical stimuli as well as thermal stimuli.TRPV4 protein expresses widely in various kinds of tissues.When it comes to the heart, T
In addition to a fast activating and immediately inactivating inward sodium current, many types of excitable cells possess a noninactivating or slowly inactivating component: the persistent sodium cur
In order to explore the modulaory effects of metabotropic glutamate receptors (mGluRs) on descending activation of spinal motoneurons (MNs), the intracellular recording techniques were conducted in MN
Background: The Kv 1.3 potassium channel serves as an important regulator of lymphocyte function.Blockade of the channel depolarizes the cell membrane and attenuates the Ca2+ signaling pathway that is