基于外切酶III和DNAzyme循环放大检测p53基因

来源 :2016全国生命分析化学学术大会 | 被引量 : 0次 | 上传用户:telecom_god0221
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  基于功能化DNAzyme 和循环放大技术设计了一种检测DNA 的新方法,通过Exo Ⅲ和功能化DNAzyme 的双重放大作用可使荧光信号增强.实验原理如图1 所示,在没有ExoⅢ 的情况下,不会产生荧光信号,只有在Exo Ⅲ 存在的情况下,才能进行三个循环反应,使荧光信号增强.在最佳条件下p53 基因的检测的线性范围为 1.0 × 10-14 M~1.0 × 10-10 M.我们还进行了错配碱基序列的检测,实验结果表明,传感器具有较好的选择性.同时,我们在实际样品中进行p53 基因的检测,荧光信号不受影响.该方案具有操作简单、灵敏度高、费用低等优点,整个检测过程可以在均相溶液完成.
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