On-site nucleic acid testing(NAT)provides a rapid,accurate and user-friendly method for pathogen diagnosis,food safety analysis and environmental monitoring [1-3].Here,we developed an automated and fully-integrated nucleic acid analyzer,by combining the on-site NAT with sequential operation droplet array(SODA)technique [4].The present analyzer could achieve multiple operations including sample introduction,nucleic acid extraction,reverse transcription-amplification,sample distribution,real-time fluorescence quantification,dual fluorescence detection.A strategy of compact and low-cost system construction was adopted to minimize the analyzer to 50 × 45 × 45 cm(length × width × height),and significantly reduce the instrument cost and single analysis cost.The analyzer mainly consists of a nucleic acid extraction module,a temperature control module,a multi-fluorescence detection module and a control module.The nucleic acid extraction module,which can be used for nucleic acid extraction and sample distribution,is composed of a liquid manipulation platform that can move in three dimensions and a precision syringe pump.The PCR amplification can be performed by the temperature control module and the generated droplet fluorescence signals can be captured by the detection module.In the analyzer,a simple and plastic-sealed chip was also designed to pre-load reagents and carry PCR reaction droplets,with the function of avoiding crosscontamination effectively.We applied the analyzer and the chip to identify eight influenza pathogens in human throat swabs,and the results of which were consistent with the colloidal gold method.