Aims: To identify the native-occurring adipate pathway in a previously engineered Thermobifida fusca B6 strain and optimize the adipic acid production of this strain on glucose and corncob.Methods and Results: Thenative-occurring adipate pathway in T.fusca B6 was identified to be the reverseadipate degradation pathway,including 5 steps: β-ketothiolase(Tfu_0875),3-hydroxyacyl-CoA dehydrogenase(Tfu_2399),3-hydroxyadipyl-CoA dehydrogenase(Tfu_0067),5-Carboxy-2-pentenoyl-CoA reductase(Tfu_1647)and succinyl-CoA synthetase(Tfu_2576,Tfu_2577).The cell lysates of T.fusca wild-type strain with the addition of the Tfu_1647 protein produced trace adipic acid,while no adipic acid was produced in the absence of this protein.The above results prove that the low expression of Tfu_1647 in the WT strain was the reason why it didnt produce any adipic acid.