【摘 要】
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Zebrafish has been used in metabolic study of drugs as a powerful tool in recent years.In this study,we make a feasible metabolism investigation of five protoberberine alkaloids (PBAs) applied in zebr
【机 构】
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Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing,China;Capita
【出 处】
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中国中医科学院建院60周年国际学术发展大会
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Zebrafish has been used in metabolic study of drugs as a powerful tool in recent years.In this study,we make a feasible metabolism investigation of five protoberberine alkaloids (PBAs) applied in zebrafish model for the first time, including berberine (BBR), palmatine (PAL), jatrorrhizine (JAT), coptisine (COP)and epiberberine (EBBR).2.After exposure for 24 hours, 19 metabolites were identified by LTQ Orbitrap mass spectrometer, including 9 phase Ⅰ metabolites and 10 phase Ⅱ metabolites.Demethylation,hydroxylation, sulfation and glucuronidation were the major metabolic transformation of PBAs in zebrafish,which were similar to mammals.
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