【摘 要】
：
沙门菌是寄生在人和动物肠道内可引起多种疾病的肠道致病菌,主要经水和食物传播.沙门菌质粒毒力基因(spy)与细菌毒力密切相关,其毒力表型主要由调节基因spvR、效应基因spvB和spvC介导.已知spvB的编码产物具有ADP核糖基转移酶活性,可诱导细胞骨架解聚.本课题组前期研究发现spvB可抑制宿主细胞自噬体的形成,但相关的分子机制尚不清楚.本研究第一部分以鼠伤寒沙门菌野生株、spvB基因突变株和回
【机 构】
：
苏州大学医学部基础医学与生物科学学院,江苏省苏州市工业园区仁爱路199号
论文部分内容阅读
沙门菌是寄生在人和动物肠道内可引起多种疾病的肠道致病菌,主要经水和食物传播.沙门菌质粒毒力基因(spy)与细菌毒力密切相关,其毒力表型主要由调节基因spvR、效应基因spvB和spvC介导.已知spvB的编码产物具有ADP核糖基转移酶活性,可诱导细胞骨架解聚.本课题组前期研究发现spvB可抑制宿主细胞自噬体的形成,但相关的分子机制尚不清楚.本研究第一部分以鼠伤寒沙门菌野生株、spvB基因突变株和回补株分别感染HeLa细胞,建立体外细胞感染模型,通过自噬通量和自噬体形成过程与细胞骨架的相互作用为切入点,探究spvB影响自噬体形成的分子机制.
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