Phytocyanins (PYCs), a family of small copper-containing electron transfer proteins, are specifically found in plants.Many PYC genes possess arabinogalactan-protein like regions (ALR) and therefore term chimeric arabinogalactan-proteins (CAGPs).Previously, 38 AtPYC and 62 BrPYC genes have been identified in Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa), respectively.Here, we identified 84 putative PYC genes in the annotated genome of Chinese cabbage (B.rapa ssp.Pekinensis line Chiifu-401-42).Based on motif constitution, all of these BrPYCs could be divided into three main subclasses: 51 early nodulin-like proteins (ENODLs), 16 uclacyanin-like proteins (UCLs), and 11 stellacyain-like proteins (SCLs).Structure prediction indicated that 71 BrPYCs were with N-terminal secretion signal and 45 BrPYCs were glycosylphosphatidylinositol -anchored to the plasma membrane.Glycosylation prediction revealed that 48 BrPYCs were CAGPs with putative arabinogalactan (AG) glycomodules and 57 BrPYCs had N-glycosylation sites.Additionally, gene duplication analysis uncovered that all BrPYC genes with duplication relationship shared the same conserved collinear blocks (CCB) and chromosome segment were the main reason for expansion of this gene family.Amazingly, almost all BrUCL genes were duplicated except for BrUCL16, and only BrSCL3 and BrSCL4 as a pair were tandem duplicated.Expression analyses based on large-scale publicly available UniGene information indicated that the ESTs of 25 BrPYC genes appear to be exclusively detected in specific tissue, including ESTs for 8, 9, 4, and 4 BrPYC genes are only presented in bud, flower, root, and silique, respectively.Interestingly, the BrPYC ESTs may not be existed in seed except for some of BrPYC with no available UniGene information.The genome-wide identification and bioinformatic analysis of BrPYCs in B.rapa will pave the way for studying their biological functions in Brassica species.