【摘 要】
：
Cyanidin-3-O-β-glucoside (CAS No.7084-24-4), a typical anthocyanin pigment that exists in the human diet,has been reported to have anti-inflammatory properties.However, the effect of C3G on LPS-induce
【机 构】
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Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University,Changch
【出 处】
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中国畜牧兽医学会家畜内科学会2014年学术研讨会
论文部分内容阅读
Cyanidin-3-O-β-glucoside (CAS No.7084-24-4), a typical anthocyanin pigment that exists in the human diet,has been reported to have anti-inflammatory properties.However, the effect of C3G on LPS-induced mastitis and the molecular mechanisms has not been investigated.In this study, we detected the protective effects of C3 G on LPS-induced mouse mastitis model and investigated the molecular mechanisms in LPS-stimulated mouse mammary epithelial cells.Our results showed that C3G could attenuate mammary histopathologic changes,and myeloperoxidase (MPO) activity;inhibit TNF-α, IL-1 βand IL-6 production;caused by LPS.Meanwhile, C3G dose-dependently inhibited TNF-α and IL-6 in LPS-stimulated mouse mammary epithelial cells.C3G suppressed LPS-induced NF-κB and IRF3 activation.Furthermore, C3 G disrupted the formation of lipid rafts by depleting cholesterol.Moreover, C3 G activated LXR-ABCGl-dependent cholesterol efflux.Knockdown of LXRα abrogated the anti-inflammatory effects of C3G.In conclusion, C3G has a protective effect on LPS-induced mastitis.The promising anti-inflammatory mechanisms of C3G is associated with up-regulation of the LXRα-ABCG1 pathway which result in disrupting lipid rafts by depleting cholesterol, thereby suppressing TLR4 mediated NF-κB and IRF3 signaling pathways induced by LPS.
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