【摘 要】
：
Two 12 kDa FK506-binding protein (FKBP12) genes were isolated and characterized from Chinese oak silkworm Antheraea pernyi,an important agricultural and edible insect,designated as ApFKBP12 A and B,re
【机 构】
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辽宁省昆虫资源工程技术研究中心,生物科学与技术学院,沈阳农业大学,沈阳 110866 辽宁省昆虫资
【出 处】
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第十届家(柞)蚕遗传育种及良种繁育学术研讨会
论文部分内容阅读
Two 12 kDa FK506-binding protein (FKBP12) genes were isolated and characterized from Chinese oak silkworm Antheraea pernyi,an important agricultural and edible insect,designated as ApFKBP12 A and B,respectively.Both ApFKBP12 A and B contained 108 amino acids with 82% sequence identity.Phylogenetic analysis showed that FKBP12 B sequences of A.pernyi,B.mori and D.plexippus were clearly separated from FKBP12 A sequences of these three species,suggesting that insect FKBP12 A and B may have been evolving independently.RT-PCR analyses revealed that two ApFKBP12 genes were expressed during the four developmental stages and in all tested tissues,and that the mRNA expression level of the ApFKBP12 A gene was significantly higher than the ApFKBP12 B gene.After heat shock treatment,expressions of the two FKBP12 genes were up-regulated,but at different time points.Our results suggested that each paralogue of the FKBP12 genes may play a distinct functional role in the development of A.pernyi.
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