【摘 要】
：
"性的进化和维持问题"是进化生物学问题中的女王(the queen of problems in evolutionary biology),有性生殖的优势被认为是因为其可以比无性生殖更快地适应新环境,但这种适
【机 构】
：
中国科学院水生生物研究所水生生物多样性与保护重点实验室,武汉,430072;中国科学院大学,北京,100049中国科学院水生生物研究所水生生物多样性与保护重点实验室,武汉,430072西北师范大学生命
【出 处】
：
中国动物学会原生动物学分会第十八次学术讨论会
论文部分内容阅读
"性的进化和维持问题"是进化生物学问题中的女王(the queen of problems in evolutionary biology),有性生殖的优势被认为是因为其可以比无性生殖更快地适应新环境,但这种适应优势的分子基础以及与无性生殖的具体异同均不清楚.利用实验进化基因组学的方法通过基因组转录组测序,围绕单细胞真核模式生物——嗜热四膜虫无性生殖种群和有性生殖种群在连续传代过程中对渗透压提高环境的分子适应机制展开研究.实验按对照组/处理组、无性生殖/有性生殖各5个重复共20个细胞系设计,目前无性生殖组已连续传代1100余代,有性生殖组已完成11次有性生殖周期.主要结果包括:1)通过考察连续传代过程中的生长速率,发现对照组和处理组中的有性生殖种群生长速率都要快于无性生殖种群,并且有性生殖种群间表现出更大的遗传多样性;2)利用高通量测序数据,建立了针对嗜热四膜虫SNP calling的方法.3)有性生殖种群能够产生比无性生殖种群更多的new mutation,有助于加快其适应新环境的速度.4)通过追踪对照组有性和无性种群连续传代过程中SNP频率变化信息,筛选到可能与其生长速率加快相关的"有益突变".对"有益突变"上下游基因进行功能分析发现,其功能主要涉及跨膜运输、蛋白激酶、细胞分裂和DNA复制等方面,进一步证明这些"有益突变"可能与细胞生长加快这一表型相关.
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