Brassinosteroids (BRs) were plant growth regulators, and played important roles in plant development.The function analysis of BR-regulating protein was the theory basis of clarifying how BR regulating plant growth and development.In this study, we identified some BR-regulated proteins using two-dimensional polyacrylamide gel electrophoresis, One of them is Nucleoside Diphosphate Kinase1 (OsNDPK1).