Recently,our group invented a new spectroscopic methodology called "shell-isolated nanoparticle-enhanced Raman spectroscopy"(SHINERS),which has been employed to overcome the long-term material-and substrate-specific drawbacks in conventional surface-enhanced Raman spectroscopy(SERS).Shell-isolated Ag nanoparticles inherit the merit of acquiring outstanding Raman signal; meanwhile,the concept of SHINERS could be applicable for the ultra-sensitive fluorescence analysis.In the present work,a sub-monolayer of Ag SHINs are simply covered onto the fluorescence film and coupled with Ag substrate to achieve an exceptional enhancement of emission intensity.With Ag nanomaterials,it is convenient to perform surface-enhance fluorescence at a wide range from 488 to 785 nm.Astonishingly,in the presence of Ag SHINs,all the maximum enhancements of diverse fluorophores are more than 4 orders of magnitude and the enhancement up to 1.2 x 105 fold is achieved under 633 nm excitation.This ultra-high emission enhancement could significantly facilitate the single-molecule detection.