Cell cycle control is often dysregulated in cancer cells.DNA contents and cell cycle related proteins are a key to understanding cancer biology.We have established a microfluidic image cytometry (MIC) technology for convenient cell cycle analysis of small amount of cells, MIC technology was able to distinguish DNA content changes during cell cycles with as less as a few hundreds of cells.Future development of DNA content measurement and cell-cycle specific protein imaging will enable precise cell cycle determination.This method was considered as a complementary method of flow cytometry for small amount of cells.