【摘 要】
：
Haematopoietic necrosis of gibel carp(Carassius auratus gibelio)is caused by cyprinid herpesvirus 2(CyHV-2)and has caused huge economic losses in aquacultur
【机 构】
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YangtzeRiverFisheriesResearchInstitute,ChineseAcademyofFisherySciences;Wuhan,Hubei430223,PRChinaRese
【出 处】
：
湖北省细胞生物学学会2015年会员代表大会暨学术研讨会
论文部分内容阅读
Haematopoietic necrosis of gibel carp(Carassius auratus gibelio)is caused by cyprinid herpesvirus 2(CyHV-2)and has caused huge economic losses in aquaculture worldwide.Currently the isolation and propagation of CyHV-2 in vitro is very difficult due to the lack of permissive cell lines.Studies on the pathogenesis of CyHV-2 have been hampered because the virus has not been extensively characterized.In this study,a novel cell line from the brain of gibel carp,denoted GiCB,has been established and characterized.Sustainable propagation of CyHV-2 in the GiCB cell line has been confn-med by virus infection and titration,PCR,transmission electron microscopy,immunofluorescence assay and fluorescence in situ hybridization.The GiCB cells showed typical cytopathic effect by day 6 post-infection with CyHV-2 including cell shrinkage,rounding,and cell fusion with cytoplasmic vacuolization.
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