Simultaneous determination of eight bufadienolides in cinobufacini injection by HPLC coupled with tr

来源 :中国中医科学院建院60周年国际学术发展大会 | 被引量 : 0次 | 上传用户:louism123
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A rapid and validated method was established for the simultaneous determination of eight active and toxic bufadienolides in cinobufacini injection using high performance liquid chromatography coupledwith triple quadrupole mass spectrometry.These eight compounds were separatedwithin 3 min on a C18 analytical column with gradient elution.Eleven batches of cinobufacini injection were analyzed with good linear regression relationship (r, 0.9979-0.9999), precisions (RSD, 1.92-4.79%), repeatability (RSD,3.12-4.96%), stability (RSD, 2.84-4.45%), and recovery (93.96-104.89%).By using the established method, the present study offered highly sensitive, specific, and speedy determination of eight bufadienolides, which promoted the quality control investigation of cinobufacini injection greatly.
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