Astrocytes expresses HCN channel which responds to K+ and glutamate

来源 :中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the | 被引量 : 0次 | 上传用户:xulinsheng
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
  We recently found astrocytes in primary culture expressed hyperpolarization-activated cyclic nucleotide-gated (HCN) genes (HCN2 and HCN4) and the hyperpolarization-activated cation current (Ih).The expressions of HCN genes and Ih currents were different between astrocytes and neurons.Here we demonstrated that both increase and decrease in [K+]. would down-regulate the gene expression levels.Changes of osmolarity and sodium content in the medium did not affect HCN2 gene expression.Increase of [K+]. can significantly up-regulate Ih current.Glutamate treatment alone did not affect the HCN2 gene expression and Ⅰh current in astrocyte.Co-treatments of glutamate and K+ could modulate the effect of K+ to the HCN2 gene expression and the glutamate effect was mediated by AMPA and NMDA receptors.Our findings indicate that the astrocytic HCN channel could respond to K+ and glutamate,2 major factors in neuronal excitation.Therefore,HCN might play an important role in controlling the resting membrane potential of astrocytes,thus contributing to the maintenance of electrophysiological homeostasis of the brain.
其他文献
In our previous work,a rat model of tibia bone was established by inoculating the Walker 256 rat mammary gland carcinoma cells into tibia cavity.The rat showed significant bone destruction and display
Sema4C is a member of transmembrane semaphorin proteins which regulate axonal guidance in the developing nervous system.The expression of Sema4C was dramatically induced not only during differentiatio
A transcription factor known as cyclic AMP response element-binding protein has been shown to be involved in the central sensitization in neuropathic pain and inflammation pain.The present study exami
Concentration of extracellular calcium ([Ca2+]o) in the central nervous system decreases substantially in different conditions.It results in facilitating neuronal excitability.The goal of this study w
Estrogen as a natural antioxidant of membrane phospholipids peroxidation can block the damage of dopaminergic neurons in substantia nigra (SN) lesioned by exotic toxicant like 6-hydroxydopamine (6-OHD
目的 探索8p基因酸性神经酰胺酶基因(ASAH1)和神经胶质细胞生长因子-1(NRG-1)基因与精神分裂症易感性之间的关系.方法 应用聚合酶链反应和限制性片段长度多态性技术(PCR-RFLP)对254例精神分裂症患者ASAH1基因上的3个单核苷酸多态性(rs3753118、rs3753116和rs7830490)、NRG-1基因上的2个单核苷酸多态性(rs2439322和rs16879809)进行
Glial cell line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor for midbrain dopamine (DA) neurons.GDNF exerts its biologic effects through a multi-component receptor system includi
Prolactin-releasing peptide (PrRP) was isolated and identified as an endogenous hypothalamic ligand for the GPR10 (G protein-coupled receptor 10).The anatomical distribution of PrRP-immunoreactive (Pr
Our laboratory has previously reported that 14-3-3g could be specifically induced by ischemia in astrocyte and has a neuroprotective effect.We attempted to uncover the mechanism involved in this induc
Methylprednisolone (MP) has been widely used as a standard therapeutic agent for the treatment of spinal cord injury (SCI).However,its beneficial effects are controversial.Thus,the combination of MP a