Enhancement of immune activities of Brassica oleracea var.italica were investigated on follows nanoparticle of extracts associated with ultrasofication process at 60℃ and showed the high promotion of human B and T cell growth,about 7~35% compared to the control.The secretion of IL-6 and TNF-α was also enhanced as 2.56×10-4 pg/cell and 2.13×10-4 pg/cell each,by the adding nano samples on T cell growth.NK cell activation was improved up to 8% higher than the control,through adding culture medium of T cell added nano samples.It was also found that extracts from B.oleracea var.italica could have high inhibition activity of Hyaluronidase as IC50 about 200 tg/ml.It can be concluded that the samples by nano-encapsulation,each size is 200 nm,from B.oleracea var.italica has high immune activities through higher efficiency of bio-activation than conventional extracts.