【摘 要】
：
Objective In the present study, we investigated whether curcumin have therapy value in testicular cancer and examined the effects of curcumin on the amplification and expression of AP-2γ.Methods Dose-
【机 构】
：
Department of Biochemistry and Molecular Biology, Key Laboratory of Protein Chemistry and Developmen
论文部分内容阅读
Objective In the present study, we investigated whether curcumin have therapy value in testicular cancer and examined the effects of curcumin on the amplification and expression of AP-2γ.Methods Dose-response and time-course anti-proliferation effects were examined by MTT assay and colony formation assay.Hoechst 33258 staining, TUNEL analysis and Annexin Ⅴ-FITC / PI staining assay were used to analyze the cell apoptosis.Protein expression was examined using Western blot analysis or Immunocytochemical staining.Results In this study, we show that curcumin treatment resulted in a dose and time dependent inhibition of proliferation and colony formation in testicular teratocarcinoma cell lines.Furthermore, curcumin treatment induced apoptosis through the intrinsic pathway, extrinsic pathway and caspase pathway by reducing the expression of FasL and the Bcl-2-to-Bax ratio, in addition to activating the caspase-9/-8 and caspase-3 cascades.Western blot and Immunocytochemical staining showed that curcumin induced a concentration-dependent reduction of AP-2γ expression and this reduction was due to proteosome-mediated degradation of this protein.Additionally, inhibition of AP-2γ induced changes in NTera-2 cells similar to those in NTera-2 cells treated with curcumin.Furthermore, we observed that curcumin inhibited the expression of ErbB2, and the phosphorylation of Akt and MAPK was decreased, whereas there were no decrease in total ERK and AKT protein levels in NTera-2 cells.Conclusion Taken together, these findings for the finst time provide evidence that curcumin significantly induces apoptosis and inhibits the cell proliferation of NTera-2 cell may be through inhibiting AP-2γ downstream cell survival signaling pathways.
其他文献
高通量测序又称为第二代测序，相比于第一代以Sanger为代表的测序技术，具有通量高、产量高、准确度高、分析自动化等特点，近些年随着高通量测序技术的发展，其应用范围也逐步扩大到医疗、农业及环境等领域。序列捕获是采用高密度合成的探针，将基因组上感兴趣的部分通过碱基互补加以富集的过程。因为多数捕获区位于基因的外显子区，因此又常常被称为外显子捕获。
Objective A recent genome-wide meta-analysis identified six new susceptible genetic variants for non-syndromic orofacial clefts (NSOC).The aim of the current study was to explore whether these newly i
目的 Notch1胞内段荧光表达载体的构建及鉴定.方法 提取人高分化鼻咽癌细胞CNE1株的RNA；通过RT-PCR获得Notch1胞内段(NIC)的编码序列,通过基因重组技术将其插入到pEGFP-N1中；利用酶切和测序确定重组质粒pEGFP-N1-NIC的正确性；采用脂质体介导的方法将重组质粒转染到人低分化鼻咽癌细胞CNE2中,通过荧光显微镜观察、RT-PCR和Western blot的方法检测绿
CYP21基因是先天性肾上腺皮质增生症的致病基因,90％～95％的21-羟化酶缺乏症患者在CYP21基因上存在有害突变.了解CYP21基因编码区的常见突变谱和突变热点,同时分析基因型与表现型的关系,有着重要的意义.来自美国在内的13个国家的650万新生儿数据显示,其发病率为1/15000,经典型人群携带率为1/60,失盐型人群携带率为67％,非失盐型人群携带率为33.2％,这些数据在某些人种中可能
目的 探讨一种简单、快捷的不依赖于载体多克隆位点及目的序列的基因亚克隆PCR方法.方法 设计一对针对目的序列及载体序列的PCR上、下游引物,扩增目的序列的引物末端分别和载体序列有18个碱基的重叠,使用高保真的DNA聚合酶进行目的序列和载体序列的扩增,扩增产物混合后,利用DpnI消化降解目的序列及载体的PCR模板,混合物直接用于转化,挑取阳性克隆,测序证明方法的可靠性,提取阳性克隆的质粒,体外转录的
Background Hereditary spastic paraplegia (HSP) is a clinically and genetically heterogeneous neurodegenerative diseases characterized by progressive spasticity of the lower limbs.The various mutations
目的 疼痛是在人体受到各种伤害性刺激时所产生的感觉,神经性疼痛一直是困扰医学界的难题,发病机理不是十分清楚.本研究收集并鉴定两个发作性神经疼痛遗传病大家系,通过研究希望发现神经性疼痛的致病基因,探讨其发病的分子机制.方法 采用微卫星标记的全基因组连锁分析、外显子组和Sanger测序、RFLP技术及电生理学方法.结果 选取收集的两个常染色体显性遗传的疼痛家系的先证者,对其进行直接测序分析排除已知的三
目的 探讨云南傣族和布朗族α和β地中海贫血的基因突变谱特征.方法 对随机获得的云南西双版纳傣族自治州的403份傣族和174份布朗族血液样本，通过血常规和血红蛋白电泳检测地中海贫血表型，采用单管多重Gap-PCR、联合ARMS法及DNA测序技术对地中海贫血基因突变类型进行检测.结果 403例傣族中，α、β地贫基因携带率分别为24.32％和6.45％； 174例布朗族中，α、β地贫基因携带率为分别为3
染色体不稳定综合征亦称染色体脆性综合征或染色体断裂综合征，其主要特征是由于染色体稳定性受到破坏进而产生一系列临床与细胞遗传学改变。一些疾病由于DNA修复酶缺陷导致染色体不稳定，易发生断裂、缺失、重排等现象。染色体不稳定综合症患者除易患白血病或恶性肿瘤外，同时还有全身染色体容易断裂或对紫外线特别敏感的特点。染色体不稳定综合征分为"典型的染色体不稳定综合征"和"新的染色体不稳定综合征"两大类，典型不稳
研究背景 假肥大性进行性肌营养不良又称Duchenne型肌营养不良(Duchenne Muscular Dystrophy,DMD,OMIM 310200)是常见的X连锁隐性遗传病,发病率为1/3500活产男婴,女性多为致病基因的携带者.通常5岁左右发病,20岁左右死于呼吸衰竭等严重并发症,目前尚无特效治疗措施,预后差. 1987年,Hoffman等人利用免疫学方法确认了抗肌萎缩蛋白(Dystro