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Microalgae is acknowledged as a kind of promising non-food biomass for fuel and chemicals production owing to its high photosynthetic efficiency, by which light, carbon dioxide and other nutrients could be converted to lipids, carbohydrates and proteins.Among vast microalgae species, Chlorella, which could be easily and fast cultivated, has been considered as a promising feedstock for large scale cultivation to produce biofuel and value added chemicals.Reports have focused on various issues of Chlorella, including different trophic modes for efficient biomass growth, the application of N-deficiency culture for increasing calorific values and, the regulation of iron concentration for higher lipid content.In this work, different light intensities (80, 160, 240 and 320 μmol/m2s) and various mediums including control medium (CM), N/P rich medium (NPM), N rich medium (NM), and P rich medium (PM) were applied for cultivation of Chlorella sp.It was revealed that cultivation of Chlorella sp.in CM under the light intensity of 320 μmol/m2s led to a lipid content up to 30% enhancement, which was higher than the results of other cases.A rather high unsaturated fatty acid (UFA) content of 7.5% and unsaturated fatty acid/total fatty acid (UFA/TFA) ratio of 0.73 were obtained under 320 μrol/m2s in CM, indicating that the CM-320 system was applicable for the generation of UFA.Moreover, Chlorella sp.cultivated in PM under 320 μmol/m2s provided higher TFA content (7.3%), which was appropriate for biofuel production.