【摘 要】
：
目的：探讨脂联素对人乳腺癌细胞T47D细胞周期以及凋亡所产生的影响,以及血清脂联素水平与乳腺癌发生风险之间的关系及发生中的作用.方法：选择山西省肿瘤医院乳腺外科2009年1月到2010年1月收治的术前女性乳腺癌原发病例100例,按照频数匹配的方法选择病人陪侍100例作为对照组,进行血清学检测.对数生长期的乳腺癌细胞T47D细胞系,检测0,500ng/ml,2000 ng/ml脂联素对其细胞周期及凋
【机 构】
：
030013 太原,山西省肿瘤医院病因室 山东省济宁市第一人民医院
【出 处】
：
2012年全国肿瘤分子标志学术大会与国际肿瘤转化医学论坛暨第七届中国中青年肿瘤专家论坛
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目的：探讨脂联素对人乳腺癌细胞T47D细胞周期以及凋亡所产生的影响,以及血清脂联素水平与乳腺癌发生风险之间的关系及发生中的作用.方法：选择山西省肿瘤医院乳腺外科2009年1月到2010年1月收治的术前女性乳腺癌原发病例100例,按照频数匹配的方法选择病人陪侍100例作为对照组,进行血清学检测.对数生长期的乳腺癌细胞T47D细胞系,检测0,500ng/ml,2000 ng/ml脂联素对其细胞周期及凋亡的影响.结果：(1)乳腺癌组脂联素水平(6.01±1.98)u g/ml明显低于健康对照组(11.56±3.36)u g/ml,差异有显著性(P＜0.01)；乳腺癌组脂联素水平在绝经前与绝经后比较差异均有显著性(分别t=3.48,P＜0.01).(2)脂联素对T47D细胞周期的影响,脂联素浓度为500ng/ml,2000 ng/ml时,G0/G1期总体差异有统计学意义(F=29.30,P=0.011),S期及G2/M期差异无统计学意义(F=3.97,P=0.144；F=0.25,P=0.82),但两组浓度间无显著性差异.(3)脂联素对T47D细胞凋亡的影响,结果显示,0,500ng/ml,2000ng/ml脂联素作用24h后,T47D细胞的早期凋亡率分别为(18.52±0.43)％,(18.75±0.11)％,(18.78±0.30)％, 无统计学差异(F=0.419,P=0.691),总凋亡率分别为(24.58±2.25)％,(24.69±0.27)％,(24.11±0.87)％,无统计学差异(F=0.099,P=0.909).结论：(1)血清脂联素水平降低,与乳腺癌的发生相关.乳腺癌组脂联素水平绝经前明显低于绝经后,提示体内激素水平可能也参与乳腺癌的发生.(2)脂联素可以将T47D细胞周期阻滞在G0/G1期,使S期细胞数量下降,抑制肿瘤细胞DNA合成.
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