【摘 要】
：
The aim of the present study was to investigate effects of Se deficiency (SeD) on the mRNA expression of inflammatory factors and 25 selenoprotein genes in kidney of broiler chicks.150 1-day-old broil
【机 构】
：
Northeast Agricultural University, Harbin 150030, P.R.China;Heilongjiang Polytechnic, Harbin 150080,
【出 处】
：
中国畜牧兽医学会兽医内科与临床诊疗学分会第八届代表大会暨学术研讨会
论文部分内容阅读
The aim of the present study was to investigate effects of Se deficiency (SeD) on the mRNA expression of inflammatory factors and 25 selenoprotein genes in kidney of broiler chicks.150 1-day-old broiler chicks were randomly assigned to two groups fed with a low-Se diet (SeD group,0.033 mg/kg Se)or adequate Se diet(C group,0.2 mg/kg Se).The levels of uric acid (UA)and creatinine (Cr)in the serum,the mRNA levels of 6 inflammatory factors and 25 selenoprotein genes in kidney were determined as the symptom of SeD occurred.The results showed that the contents of UA and Cr in the serum were significantly increased in the SeD group.The mRNA levels of inflammatory factors (NF-κB, iNOS, COX-2, TNF-α)were significantly increased in SeD group.Meanwhile, the mRNA levels of PTGEs and HO-1 were not significantly changed in SeD group.In addition,25 selenoprotein transcripts displayed a ubiquitous expression in kidney of chicks.The mRNA levels of 14 selenoprotein genes (Dio1, Dio2, GPx3, Sepp1, SelH, SelI, SelK, Sepn1, SelO, SelW, Sel15, SelT, SelU and SelS)were decreased and 9 selenoprotein genes (GPx1, GPx2, GPx4, SelPb, TrxR1, TrxR2, TrxR3, SPS2 and SelM)were up-regulated in the SeD group,but the changes of Dio3 and Selx1 mRNA levels were not significant (P> 0.05).The results indicated that Se deficiency induced the abnormal renal functions.The change of the inflammatory factors mRNA played an important role in the abnormal renal functions induced by SeD.25 selenoprotein genes showed a different expression in SeD group.
其他文献
Long-term consumption of high concentrate diet causes the accumulation of organic acid and a depression of pH values in rumen.When rumen pH decreased below 5.6 at least 3 hours one day, subacute rumin
Dairy cows are often fed a high-concentrate diet to meet lactating demands, yet long-term concentrate feeding induces subacuteruminal acidosis (SARA) and leads to a decrease in milk fat.The liver is r
Dairy cows are often fed high concentrate diet (HC) to meet the nutritional demands of lactation.However, overfeeding cattle with a diet rich in concentrate for long-term results in a metabolic disord
Sub-acute ruminal acidosis (SARA), which is also known as chronic or sub-clinical acidosis, is a comm on health problem in many dairy herds, where high concentrate is largely used to counteract negati
[Backgroud]γ-oryzanol is one of the most important bioactive substances in rice bran oil that can be used for food, pharmaceutical and cosmetic fields.To investigate the proliferative and protective e
In the dairy industry, it is currently a common practice to feed a high concentrate (HC) diet to lactating cows to meet their energy requirements to support high milk production.However, the feeding o
Salmonella was a worldwide important pathogenic bacteria, belonged to the enterobacteriaceae family,Salmonella genus and the facultative anaerobic gram-negative bacilli.Salmonella had the general flag
[Objective]The study was to investigate the effects of the complex immune stress induced by Newcastle Disease Ⅳ Series and lipopolysaccharides (LPS) on the growth performance and antioxidant capacity
[Backgroud]Dexamethasone (Dex), a synthetic glucocorticoid, is strictly controlled for use due to its serious side effects including immune suppression.Betulinic acid (BA), an antioxidant prepared fro
Animals and humans with severe leptospirosis may require empirical treatment.Although many antibiotics are active against multiple leptospira serovars in vitro, their efficacy in vivo is limited.We ev