【摘 要】
：
In most fruit,chlorophyll degradation occurs during development or in postharvest storage.It is particularly important in citrus fruit,where postharvest degreening is an important issue,and this speci
【机 构】
：
College of Agriculture & Biotechnology/Zhejiang Provincial Key Laboratory of Horticulture Plant Inte
【出 处】
：
2015 International Conference on Fruit Quality Biology (第二届果
论文部分内容阅读
In most fruit,chlorophyll degradation occurs during development or in postharvest storage.It is particularly important in citrus fruit,where postharvest degreening is an important issue,and this specific process is proving particularly useful for investigating mechanisms of chlorophyll degradation.However,transcription factors that directly regulate chlorophyll degradation,have been rarely reported.CitERF13,previously identified as a one of the CitERF genes with particular potential for regulating chlorophyll degradation,was characterized as having a significant correlation with chlorophyll degradation in citrus fruit peel.Dualluciferase assays indicated that CitERF13 trans-activated the promoter of CitPPH,further experiments indicated that the DRE motif in the CitPPH promoter is critical for such trans-activation,and yeast one-hybrid assays indicated that CitERF13 and CitPPH promoters had physical interaction.Transient over-expression of CitERF13 in Nicotiana tabacum leaves led to leaf yellowing and lower chlorophyll content,and an increase in endogenous PPH gene expression,pheophorbide(pheide)a content(metabolite of PPH)and ROS.The effects of CitERF13 on chlorophyll degradation were also tested in the navel orangeNewhalland mandarin Ponkan,along with stable over-expression in N.tabacum.Based on these results,it was proposed that CitERF13 was an direct transcriptional activator on chlorophyll degradation,and the activation effects were manifested by DRE motif and were conducted via DNA-protein interaction with CitPPH.
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